Different sample preparation methods combined with LC-MS/MS and LC-UV for determination of some furocoumarin compounds in products containing citruses
Identifieur interne : 002229 ( Main/Exploration ); précédent : 002228; suivant : 002230Different sample preparation methods combined with LC-MS/MS and LC-UV for determination of some furocoumarin compounds in products containing citruses
Auteurs : Helena Prosen [Slovénie] ; Drago Kocar [Slovénie]Source :
- Flavour and fragrance journal [ 0882-5734 ] ; 2008.
Descripteurs français
- Pascal (Inist)
- Analyse quantitative, Electrospray, Dérivé de la furocoumarine, Dérivé du psoralène, Extraction SPE, Cosmétique, Citrus, Bergaptène, Extraction liquide liquide, Huile essentielle, Pelure, Fruit, Thé, Jus fruit, Pomélo, Méthode couplée, Chromatographie HPLC, Détecteur UV, Enrichissement chimique, Extraction solvant, Analyse trace, Analyse chimique, Ionisation chimique, Pression atmosphérique, Préparation échantillon, Camellia sinensis, LC MS MS, LC UV, APCI, Bergamottine, Citrus bergamia, Thé aromatisé, Spectrométrie masse tandem.
- Wicri :
- topic : Analyse quantitative, Huile essentielle, Fruit, Thé.
English descriptors
- KwdEn :
- Atmospheric pressure, Bergapten, Camellia sinensis, Chemical analysis, Chemical enrichment, Chemical ionization, Citrus, Cosmetic, Coupled method, Electrospray, Essential oil, Fruit, Fruit juice, Furocoumarine derivatives, Grapefruit, HPLC chromatography, Liquid liquid extraction, Peel, Psoralen derivatives, Quantitative analysis, Sample preparation, Solid phase extraction, Solvent extraction, Tandem mass spectrometry, Tea, Trace analysis, Ultraviolet detector.
Abstract
Furocoumarins are a group of naturally occurring oxygen heterocyclic compounds synthesized by different plants (mainly Citrus species). They have several physiological etiects, e.g. photoreactivity and inhibition of intestinal and liver drug metabolism, which imply the need to control their concentration in products intended for human use. We tested different sample preparation approaches and LC with different detection modes: UV, APCI-MS/MS and ESI-MS/MS for the determination of bergapten (BGPT) and bergamottin (BGMT) in different samples of beverages and cosmetics. Direct injection of sample solutions was applicable only for essential oils, while liquid-liquid extraction proved to be time-consuming and unsuitable for our samples. Solid-phase extraction with a preconcentration factor of 20 yielded repeatable recoveries for aqueous solutions (97.4% for BGPT, 64.6% for BGMT) and the concentration range was > 0.1 mg/l for UV detection and 2-1000 μg/l for MS/MS detection. Results of sample analysis indicated some problems connected both to detection and the extraction. The standard addition method for quantification yielded the most reliable results.
Affiliations:
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Le document en format XML
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citruses</title><author><name sortKey="Prosen, Helena" sort="Prosen, Helena" uniqKey="Prosen H" first="Helena" last="Prosen">Helena Prosen</name><affiliation wicri:level="1"><inist:fA14 i1="01"><s1>Faculty of Chemistry and Chemical Technology, University of Ljubljana, Askerceva 5</s1><s2>1000 Ljubljana</s2><s3>SVN</s3><sZ>1 aut.</sZ><sZ>2 aut.</sZ></inist:fA14><country>Slovénie</country><wicri:noRegion>1000 Ljubljana</wicri:noRegion></affiliation></author><author><name sortKey="Kocar, Drago" sort="Kocar, Drago" uniqKey="Kocar D" first="Drago" last="Kocar">Drago Kocar</name><affiliation wicri:level="1"><inist:fA14 i1="01"><s1>Faculty of Chemistry and Chemical Technology, University of Ljubljana, Askerceva 5</s1><s2>1000 Ljubljana</s2><s3>SVN</s3><sZ>1 aut.</sZ><sZ>2 aut.</sZ></inist:fA14><country>Slovénie</country><wicri:noRegion>1000 Ljubljana</wicri:noRegion></affiliation></author></analytic><series><title level="j" type="main">Flavour and fragrance journal</title><title level="j" type="abbreviated">Flavour fragr. j.</title><idno type="ISSN">0882-5734</idno><imprint><date when="2008">2008</date></imprint></series></biblStruct></sourceDesc><seriesStmt><title level="j" type="main">Flavour and fragrance journal</title><title level="j" type="abbreviated">Flavour fragr. j.</title><idno type="ISSN">0882-5734</idno></seriesStmt></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Atmospheric pressure</term><term>Bergapten</term><term>Camellia sinensis</term><term>Chemical analysis</term><term>Chemical enrichment</term><term>Chemical ionization</term><term>Citrus</term><term>Cosmetic</term><term>Coupled method</term><term>Electrospray</term><term>Essential oil</term><term>Fruit</term><term>Fruit juice</term><term>Furocoumarine derivatives</term><term>Grapefruit</term><term>HPLC chromatography</term><term>Liquid liquid extraction</term><term>Peel</term><term>Psoralen derivatives</term><term>Quantitative analysis</term><term>Sample preparation</term><term>Solid phase extraction</term><term>Solvent extraction</term><term>Tandem mass spectrometry</term><term>Tea</term><term>Trace analysis</term><term>Ultraviolet detector</term></keywords><keywords scheme="Pascal" xml:lang="fr"><term>Analyse quantitative</term><term>Electrospray</term><term>Dérivé de la furocoumarine</term><term>Dérivé du psoralène</term><term>Extraction SPE</term><term>Cosmétique</term><term>Citrus</term><term>Bergaptène</term><term>Extraction liquide liquide</term><term>Huile essentielle</term><term>Pelure</term><term>Fruit</term><term>Thé</term><term>Jus fruit</term><term>Pomélo</term><term>Méthode couplée</term><term>Chromatographie HPLC</term><term>Détecteur UV</term><term>Enrichissement chimique</term><term>Extraction solvant</term><term>Analyse trace</term><term>Analyse chimique</term><term>Ionisation chimique</term><term>Pression atmosphérique</term><term>Préparation échantillon</term><term>Camellia sinensis</term><term>LC MS MS</term><term>LC UV</term><term>APCI</term><term>Bergamottine</term><term>Citrus bergamia</term><term>Thé aromatisé</term><term>Spectrométrie masse tandem</term></keywords><keywords scheme="Wicri" type="topic" xml:lang="fr"><term>Analyse quantitative</term><term>Huile essentielle</term><term>Fruit</term><term>Thé</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">Furocoumarins are a group of naturally occurring oxygen heterocyclic compounds synthesized by different plants (mainly Citrus species). They have several physiological etiects, e.g. photoreactivity and inhibition of intestinal and liver drug metabolism, which imply the need to control their concentration in products intended for human use. We tested different sample preparation approaches and LC with different detection modes: UV, APCI-MS/MS and ESI-MS/MS for the determination of bergapten (BGPT) and bergamottin (BGMT) in different samples of beverages and cosmetics. Direct injection of sample solutions was applicable only for essential oils, while liquid-liquid extraction proved to be time-consuming and unsuitable for our samples. Solid-phase extraction with a preconcentration factor of 20 yielded repeatable recoveries for aqueous solutions (97.4% for BGPT, 64.6% for BGMT) and the concentration range was > 0.1 mg/l for UV detection and 2-1000 μg/l for MS/MS detection. Results of sample analysis indicated some problems connected both to detection and the extraction. The standard addition method for quantification yielded the most reliable results.</div></front></TEI><affiliations><list><country><li>Slovénie</li></country></list><tree><country name="Slovénie"><noRegion><name sortKey="Prosen, Helena" sort="Prosen, Helena" uniqKey="Prosen H" first="Helena" last="Prosen">Helena Prosen</name></noRegion><name sortKey="Kocar, Drago" sort="Kocar, Drago" uniqKey="Kocar D" first="Drago" last="Kocar">Drago Kocar</name></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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